Spin Universal RNA Mini Kit

Product Details

The InviTrap® Spin Universal RNA Mini Kit simplifies total RNA isolation from different starting materials such as cells, tissue, and blood. In addition, RUO protocols are available for e.g., RNA Cleanup from TRIzol®, simultaneous extraction of DNA and RNA or RNA extraction from other body fluids. Genomic DNA is removed without an enzymatic digestion step, and RNases are inactivated to prevent RNA degradation.

 

Specifications
Downstream application PCR techniques, NGS and hybridization methods
Target nucleic acid RNA
Starting material
  • Cell culture: up to 1 x 107 cells/ml
  • Up to 1.5 ml fresh whole blood (EDTA / citrate stabilized, but not heparin)
  • Up to 20 mg fresh or frozen tissue
Yield
  • From cell culture: up to 80 µg total RNA e.g., 10 – 15 μg total RNA from
    5 x 105 NIH 3T3 fibroblasts
  • From blood: up to 1 – 5 µg total RNA / ml of whole blood (depending on leukocyte count)
  • From tissue: up to 80 µg total RNA (depending on type and amount of starting material)
Quality A260: A280 1.8 – 2.3
Preparation time
  • Cells: approx. 20 – 25 min (incl. lysis)
  • Blood: approx. 45 min (incl. Buffer EL treatment)
  • Tissue: approx. 15 – 20 min (excl. sample homogenization)
Elution Volume 30 – 100 µl
Technology Spin column
Certification CE-IVD (in compliance with REGULATION (EU) 2017/746 on in vitro diagnostic medical devices)

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Spin Universal RNA Mini Kit
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